- Construction Zone characters
Successfully complete the indicated task to unlock the corresponding character in the Construction Zone:

Mini Pauline	Acquire all the trophies and M-Tokens in every level.
Mini DK	Successfully create and complete 99 levels in the Construction Zone.
Mini Princess	Successfully create and complete 49 levels in the Construction Zone.
Mini Toad	Create and submit a level for Nintendo's Challenge Mode contest.

- Plus mode
Successfully complete the game to unlock Plus mode.

- Sound Test option
Successfully complete the game to unlock the 'Sound Test' option.

- Expert levels
Normal and Plus modes have expert levels that are unlocked in each mode when you complete that mode's Donkey Kong Final Battle.

- Expert levels
Get the indicated number of trophies by beating the goal score in a level to unlock the corresponding special level under the 'X' tab at the level selection screen:

X-4	40 trophies
X-8	80 trophies
X-7	70 trophies
X-2	20 trophies
X-9	90 trophies
X-3	30 trophies
X-6	60 trophies
X-1	10 trophies
X-5	50 trophies
X-10	100 trophies

- Special levels
Get the indicated number of M-Tokens to unlock the corresponding special level under the 'S' tab at the level selection screen:

S-13	65 M-Tokens
S-8	40 M-Tokens
S-17	85 M-Tokens
S-18	90 M-Tokens
S-7	35 M-Tokens
S-2	10 M-Tokens
S-16	80 M-Tokens
S-3	15 M-Tokens
S-10	50 M-Tokens
S-19	95 M-Tokens
S-1	5 M-Tokens
S-11	55 M-Tokens
S-5	25 M-Tokens
S-12	60 M-Tokens
S-15	75 M-Tokens
S-9	45 M-Tokens
S-6	30 M-Tokens
S-4	20 M-Tokens
S-14	70 M-Tokens
S-20	100 M-Tokens

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