- Duke's classic costume
At the title screen or main menu, press Left, Up, Square, Up, Right, Triangle to unlock Duke's classic costume. If you entered the code correctly, you will hear a sound.

- Scarlett's classic costume
At the title screen or main menu, press Right, Up, Down(2), Triangle to unlock Scarlett's classic costume. If you entered the code correctly, you will hear a sound.

- Play as Baroness, Destro, and Firefly
Successfully complete the bonus mission to unlock Baroness, Destro, and Firefly for purchase for 15 Battle Points each.

- Trophies
Successfully complete one of the following tasks to get a trophy:

Bronze Trophies
Arctic Finishd	Finish all Arctic missions.
Sloppy Joe	Achieve the worst rank on a level without going Man Down.
It Takes Two	Finish any mission in 2 player co-op.
Classified	Acquire all Intel Cards.
Half the Battle	Acquire 50% of all File Cards.
Gimme The Keys	Drive every G.I. JOE vehicle in the game.
Triplets	Pick up all three types of Point Multipliers in one mission.
Girls Night Out	Finish any mission using female characters only.
Turret Syndrome	Operate the turret in every vehicle in the game.
Yo Joe!	Purchase two G.I. JOE contacts.
Side Mission Completist	Finish all Sub-operation Missions.
Double Barrel Roll	Perform 10 barrel rolls in unison in any level in co-op.
Go Commando	Finish a mission using only Commando class characters.
Combat Rock	Finish a mission using only Combat class characters.
Firefly	Overcome Firefly.
Hack 'n Slash	Finish a level using only melee attacks on ground troops.
Man Up	Finish a Jungle or Tundra mission without going Man Down.
Bevvy o' Heavy	Finish a mission using only Heavy class characters.
Destro	Overcome Destro.
Code Cracker	Gain access to a Combat Soldier Class Ability door.
Lovers' Quarrel	Finish any mission using Baroness and Duke.
Arishikage Attack	Finish any mission using Snake Eyes and Storm Shadow.
The Tables Have Turned	Finish any Arctic, Desert, or Jungle mission using any COBRA character.
Lost Keys	Gain access to a Heavy Class Ability door.
File-o-phile	Acquire all File Cards.
Higher Realize	Replay a mission and earn a higher score.
Strike Lieutenant	Overcome every wave in one Satellite Strike mini-game.
Baroness	Overcome Baroness.
Nimble Access	Gain access to a Commando Class Ability door.
Lost & Found	Acquire all G.I. JOE Contacts.
Silver Trophies
Jungle Finishd	Finish all Jungle missions.
Battle Master	Acquire and spend all 130 possible Battle Points.
Acceleration Celebration	Activate the Accelerator Suit three times in any mission.
Desert Finishd	Finish all Desert missions.
Strike Commander	Overcome every wave in all Satellite Strike mini-games.
Launch Money	Launch 50 enemies into the air and kill them..
Hardcore G.I. JOE	Finish every Mission on Hardcore Difficulty.
Storm Shattered	Overcome Storm Shadow in all three secret arenas.
Accomplished Mission	Gain the highest Mission Ranking.
Gold Trophies
M.A.S.S. Destruction	Destroy the M.A.S.S. Device.
COBRAAAA!!!	Purchase all COBRA characters.
All G.I. JOE Contacts	Purchase all G.I. JOE contacts.
Platinum Trophies
G.I. JOE	Unlock all trophies.

- Bonus mission
Successfully complete the game. After the credits finish, a cutscene will begin. A bonus mission will now be available to play.