- Play as Archer
Successfully complete Rin's storyline to unlock Archer.

- Play as Shinji
Successfully complete Sakura's storyline to unlock Shinji.

- Play as Soichiro
Successfully complete Caster's storyline to unlock Soichiro.

- Play as Dark Sakura
Successfully complete Ilya's storyline to unlock Dark Sakura.

- Alternate costumes
Successfully complete the indicated task to unlock the corresponding alternate costume:

- Play as Taiga
Successfully complete Kirei's storyline to unlock Taiga.

- Play as Berserker
Successfully complete Saber's storyline to unlock Berserker.

- Play as Caren
Successfully complete Taiga's storyline to unlock Caren.

- Play as Kirei
Successfully complete Gilgamesh's storyline to unlock Kirei.

- Play as Gilgamesh
Successfully complete Dark Saber's storyline to unlock Gilgamesh.

- Play as Dark Saber
Successfully complete Berserker's storyline to unlock Dark Saber.

- Play as Ilya
Successfully complete Shiro's storyline to unlock Ilya.

- Play as Bazett
Successfully complete Taiga's storyline to unlock Bazett.

- Play as True Assassin
Successfully complete Rider's storyline to unlock True Assassin.

- Play as Assassin
Successfully complete Lancer's storyline to unlock Assassin.