- Play as Young Blue Jr.
Successfully complete Trial 24 to unlock Young Blue Jr.

- Play as Devil Buster Dante
Successfully complete the first level of Story mode (all four chapters) to unlock Devil Buster Dante.

- Special Story mode stages
Acquire the sixteen puzzle pieces in each stage in Story mode to unlock Special Story mode stages.

- Alternate costumes
Successfully complete all 40 Trial mode missions to unlock alternate costumes for all characters. Highlight a character at the chracter selection screen, then push Square to change their costume.

- Play as Young Captain Blue
Successfully complete Trial 40 to unlock Young Captain Blue.

- Play as Stylish Alastor
Successfully complete Trial 34 to unlock Stylish Alastor.

- Play as Fighter Bianky and Friends
Successfully complete Trial 13 to unlock Fighter Bianky and Friends.

- Play as Hulk Davidson
Successfully complete Trial 14 to unlock Hulk Davidson.

- Play as Director Blue
Successfully complete Trial 1 to unlock Director Blue.

- Play as Android Rachel
Successfully complete Trial 29 to unlock Android Rachel.

- Play as Gran Bruce
Successfully complete Trial 35 to unlock Gran Bruce.

- Play as Manager Jet
Successfully complete Trial 19 to unlock Manager Jet.

- Play as Heroine Silvia
Successfully complete Trial 37 to unlock Heroine Silvia.

- Art gallery
Acquire all sixteen puzzle pieces in each Special stage in Story mode to unlock the Art gallery.

- Play as Charles The Third
Successfully complete the second level of Story mode to unlock Charles The Third.

- Movies
Successfully complete the indicated Story mode chapter to unlock the corresponding movie:

Gulliver's Battles Special Edition	V-Puzzle 5
Truth Of The Black Film	Captain Blue Begins
Watchtiful Cartoon World	Riddle Of The Incan Empire!
Last Battle Intro Movie	Les Acteurs Incroyable
Boss*Boss*Bang*Bang	All previous chapters except The Hidden Hideout
Atlantis	Four Adventures Of The Sea
Truth Of The Black Film Special Edition	V-Puzzle 4
Gulliver's Battles	Truth Of The Black Film
Atlantis	Four Adventures Of The Sea Special Edition
Riddle Of The Incan Empire! Special Edition	V-Puzzle 1
The Hidden Hideout	Any chapter with Charles The Third, Hulk Davidson, and Gran Bruce
Captain Blue Begins Special Edition	V-Puzzle 3
Les Acteurs Incroyable	Atlantis
Captain Blue Begins	Watchtiful Cartoon World
Watchtiful Cartoon World Special Edition	V-Puzzle 2
Red Hot Rumble	Boss*Boss*Bang*Bang
Ending Movie	Les Acteurs Incroyable
Opening Movie	Riddle Of The Incan Empire!

- Play as Movie Fan Joe
Successfully complete Trial 5 to unlock Movie Fan Joe.