Successfully complete the indicated task in Story mode/Grand Prix to unlock the corresponding character signature card:
| Greiger (Matchup Mode) | Overcome Greiger in Stage 7. |
| Sunlight Unicorn | Overcome Luna in Stage 5-B. |
| Mad Archfiend | Successfully complete Story mode twice. |
| Leo's Duel Runner | Overcome Leo in Stage 2-A. |
| Officer Tetsu Trudge's Duel Runner | Overcome Officer Tetsu Trudge in Stage 4. |
| Wheel of Fortune (Jack Atlas' Duel Runner) | Successfully complete Story mode twice. |
| Facility Chief's Duel Runner | Overcome Facility Chief in Stage 3. |
| Ushi Oni | Overcome Bolt Tanner in Stage 6-B. |
| Luna's Duel Runner | Overcome Luna in Stage 5-B. |
| Officer Tetsu Trudge (Matchup Mode) | Overcome Officer Tetsu Trudge in Stage 4. |
| Security 2's Duel Runner | Be victorious in the Silver Cup in first place. |
| Normal Duel Runner (Bolt Tanner's Duel Runner) | Overcome Bolt Tanner in Stage 6-B. |
| Yusei Fudo (Matchup Mode) | Successfully complete Story mode. |
| Jack Atlas (Matchup Mode) | Successfully complete Story mode twice. |
| Summon Reactor-SK | Overcome Greiger in Stage 7. |
| Skull Acquireor | Overcome Hoodlum in Stage 1-A. |
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| Hoodlum's Duel Runner | Overcome Hoodlum in Stage 1. |
| Stardust Dragon | Overcome Story Mode once. |
| Hunter Pace (Matchup Mode) | Overcome Hunter Pace in Stage 6-A. |
| Sparks | Overcome Greiger in Stage 7 two times. |
| Bolt Tanner | Overcome Bolt Tanner in Stage 6-B. |
| Tremendous Fire | Overcome Hunter Pace in Stage 6-A two times. |
| Wall of Ivy | Overcome Akiza in Stage 5-A. |
| Burning Skull Head | Overcome Hunter Pace in Stage 6-A. |
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| Yusei's Duel Runner | Successfully complete Story mode. |
