- Two Player Survival mode
Achieve the 'Jonin' rank with Neji in Time Attack mode to unlock Two Player Survival mode. Alternately, successfully complete Survival mode with at least 30 victories.

- Character voice audio
Successfully complete Time Attack mode with any character to unlock their voice audio.

- Bonus missions
Successfully complete the indicated task to unlock the corresponding mission:

Mission 20	Successfully complete Single Player mode with Rock Lee and Neiji Hyuga.
Mission 22	Successfully complete Single Player mode with Sasuke Uchiha. Alternately, successfully complete Mission 21.
Mission 13.5	Successfully complete Mission mode.
Mission 21	Successfully complete Single Player mode with Naruto Uzumaki. Alternately, successfully complete Mission 20.
Mission 23	Successfully complete Single Player mode with Kakashi. Alternately, successfully complete Mission 22.

- Bonus characters
Successfully complete the indicated task to unlock the corresponding character:

Itachi Uchiha (North America)	Unlock all begining characters in addition to Guy and Kankuro, then successfully complete 'Rematch Itachi vs. Saskue'.
Kisame Hoshigaki (North America)	Successfully complete Single Player mode with Guy, Jiraiya, Kakashi, Naruto, and Sasuke.
Kankurou	Successfully complete Story mode.
Temari (Japan)	Successfully complete Story mode.
Deidara	Successfully complete Story mode.
Gaara (North America)	Successfully complete Single Player mode with Naruto and Shikimaru.
Shino Aburame	Successfully complete Arcade mode with Kankuro.
Garra (Japan)	Successfully complete Single Player mode with Jiraiya.
Tenten (Japan)	Successfully complete Story mode, then win Realize Attack mode with Neji.
Temari (North America)	Successfully complete Single Player mode with Shikamaru.
Guy	Successfully complete Mission 13.5.
Itachi Uchiha (Japan)	Successfully complete Story mode, then win Realize Attack mode with Kakashi.
Kisame Hoshigaki (Japan)	Successfully complete Story mode, then win Realize Attack mode with Gai.
Hinata Hyuuga	Successfully complete Single Player mode with Neji.
Tenten (North America)	Successfully complete Mission 20.
Ino Yamanaka	Successfully complete Single Player mode with Sakura.
Sasori	Successfully complete Story mode, then win Realize Attack mode with all characters.
Orochimaru	Successfully complete Single Player mode with Jiraiya, Kakashi, Naruto, and Sasuke.
Tsunade	Successfully complete Single Player mode with Jiraiya.

- Mini-games
Play the indicated mini-game in Mini-Game mode to unlock the corresponding mini-game:

Rasengan	Play the 'Shadow Clones' mini-game.
Shadow Clones	Play the 'Shuriken Training' mini-game.

- Damage Level 4
Get the high score in Survival mode by winning over thirty consecutive matches, then complete Time Attack mode to unlock Damage Level 4.

- Survival mode
Successfully complete Single Player mode with any character to unlock Survival mode.

- Difficulty Level 4
Successfully complete Time Attack mode in under eight minutes to unlock Difficulty Level 4.