- Bonus characters
Successfully complete the indicated task to unlock the corresponding character. Notice: There are multiple ways a specific character can be unlocked.

Baby Mario Unlock Baby Luigi, then save Baby Mario at the field where you battle Bowser Jr.'s team to unlock Baby Mario.
Green Shy Guy Have Peach get Green Shy Guy out of the log at Yoshi Park to unlock him.
King K. Rool Overcome all four Kritters at DK Jungle. Then, go to the waterfall, and defeat King K. Rool to unlock King K. Rool.
Funky Kong Save Funky Kong from the Hammer Brother at DK Jungle to unlock Funky Kong.
Gray Shy Guy Have Peach get Gray Shy Guy out of the log at Yoshi Park to unlock him.
Blue Pianta Get the key, and save Blue Pianta from the shop at Mario's Stadium to unlock Blue Pianta.
Daisy Successfully complete Peach's Stadium to unlock Daisy.
Green Toad Successfully complete Toadette's puzzle, then search the bush at Peach's Stadium to unlock Green Toad.
Monty Mole Use Peach on the Monty outline on the beach at Mario Stadium to unlock Monty Mole.
Pink Pianta Go to Mario Stadium to find Pink Pinata in a palm tree on the beach to unlock Pink Pianta.
Baby DK Use Yoshi's power at DK Jungle to free Baby DK from the palm tree to unlock Baby DK. Alternately, defeat DK's team.
Baby Luigi Pull the levers in Mario's Stadium to connect the bridge to unlock Baby Luigi.
Green Yoshi Free Green Yoshi at the manhole at Yoshi's ballpark to unlock Green Yoshi.
Baby Peach Use Princess Peach's heart power on the grass Toad statue at Peach Garden to unlock Baby Peach.
Red Noki Unlock and have Blue Noki and Green Noki on your team, then talk to Red Noki on the giant clam at Mario's Stadium to unlock Red Noki.
Toadette Talk to Toadette at Peach's Stadium to unlock her.
Birdo Overcome Bowser Jr. at Yoshi Park to unlock Birdo.
Light Blue Yoshi Rescue Light Blue Yoshi from Green Magikoopa in Yoshi Park to unlock him.
Tiny Kong Overcome Bowser Jr. at DK Jungle to unlock Tiny Kong.
Paragoomba Use Yoshi's ground pound on one of the palm trees in Wario City to unlock Paragoomba.
Baby Daisy Use Wario's magnet to pull Daisy’s baby rattle from the fountain at Peach Garden. Then, have Yoshi go through the manhole cover on the left side of the garden to find a crying baby. Give Baby Daisy the rattle to unlock Baby Daisy.
King Boo Talk to the Tiny Boos in a chest at Wario City to unlock King Boo. 100%; background
Green Koopa Free Green Koopa from the poster at Wario City to unlock Green Koopa.
Green Noki Unlock and have Blue Noki on your team, then talk to Green Noki at the beach at Mario's Stadium to unlock Green Noki.
Red Paratroopa Find the paintbrush at Yoshi Park, then use it to paint the giant billboard at Wario City to unlock Red Paratroopa.
Boo Have DK and Wario solve the block puzzle at Wario City to unlock Boo.
Toad Begin Peach's Stadium to unlock Toad.
Red Shy Guy Shoot the Piranha Plants at Yoshi's Ballpark to unlock Red Shy Guy.
Waluigi Find Waluigi on top of a skyscraper in Wario City to unlock him.
Paratroopa Overcome the minion guarding the lighthouse at Wario City to unlock Paratroopa.
Yellow Pianta Go to Mario Stadium to find Yellow Pinata in a barrel and unlock him.
Peach Successfully complete Peach's Stadium to unlock Peach.
Kritter Overcome King K. Rool at DK Jungle to unlock Kritter.
Blue Shy Guy Shoot the Piranha Plants at Yoshi's Ballpark to unlock Blue Shy Guy.
Red Yoshi Find the brush that Red Yoshi lost, then find him at Yoshi's Stadium to unlock Red Yoshi.
Blue Noki Successfully complete Blue Noki's task at Mario's Stadium to unlock Blue Noki.
Blooper Have Princess Peach use her heart power on the bubbles in the water at the dock in Mario Stadium to unlock Blooper. Alternately, successfully complete all tutorials in Practice mode.
Wiggler Reset the roller coaster that Wiggler is riding at Yoshi Park to unlock Wiggler. Alternately, win a game at Toy Field.
Goomba Help Goomba trace his shop at Wario City to unlock Goomba.
Yellow Yoshi Use Yoshi to get Yellow Yoshi out of a tree at Yoshi Park to unlock Yellow Yoshi.

- Error items
Unlock Wario, then use his treasure opening ability at the listed locations in Challenge mode to find the corresponding Error items:

Fire Ball	Wario City
Mini Boos	Mario Stadium
POW Ball	Peach's Ice Garden

- Minigame Madness
Successfully complete all mini-games on the Star difficulty to unlock Minigame Madness. Notice: This cannot be done in Challenge mode.

- Night mode
Be victorious in at Bowser Jr.'s Playroom in Challenge mode to unlock the ability to play during the day or night at all stadiums except Bowser's Stadium and Luigi's Mansion.

- Bonus fields
Successfully complete the indicated task in Challenge mode to unlock the corresponding field.

Bowser's Castle	Overcome Bowser in Challenge mode.
Bowser Jr. Playroom	Overcome Bowser Jr. at that stadium.
Daisy Cruiser	Buy the cruiser pass from the shop at Peach Ice Garden.
Luigi's Mansion	Buy Luigi's flashlight from the shop at Mario Stadium.

- Team characters
The following teams are made up of the indicated players:

Bowser Monsters	Bowser, Bowser Jr., Hammer Bro, Fire Bro, Boomerang Bro, Dry Bones, Green Dry Bones, Dark Bones, Blue Dry Bones, Magikoopa, Red Magikoopa, Green Magikoopa, Yellow Magikoopa
Mario Fireballs	Mario, Luigi, Blue Pianta, Red Pianta, Yellow Pianta, Blue Noki, Red Noki, Green Noki, Monty Mole, Blooper, Baby Mario, Baby Luigi
DK Wilds	DK, Diddy Kong, Dixie Kong, Tiny Kong, Funky Kong, Baby DK, King K. Rool, Kritter, Brown Kritter, Red Kritter, Blue Kritter
Wario Muscles	Wario, Waluigi, Koopa, Red Koopa, Parakoopa, Red Parakoopa, Goomba, Paragoomba, Boo, King Boo
Peach Monarchs	Peach, Daisy, Red Toad, Blue Toad, Yellow Toad, Green Toad, Purple Toad, Toadette, Toadsworth, Baby Peach, Baby Daisy, Petey Piranha
Yoshi Eggs	Yoshi, Red Yoshi, Pink Yoshi, Light Blue Yoshi, Blue Yoshi, Yellow Yoshi, Birdo, Red Shy Guy, Yellow Shy Guy, Blue Shy Guy, Grey Shy Guy, Green Shy Guy, Wiggler

- Secret Shop items
Locate the Secret Shop, then successfully complete the indicated task to unlock the corresponding item:

Star Candy	Make any purchase at the Secret Shop.
Star Candy (x2)	Purchase a Star Candy.
Super Star	Purchase three Star Candy (x2).

- Badges
Successfully complete the indicated task to unlock the corresponding badge:

Buddy	Get all characters on your team.
Error Item	Unlock all Error Items.

- Final movie
Overcome Bowser two times to unlock the last movie.