- True Story mode
Successfully complete Story mode with Dracula to unlock True Story mode. This mode has an alternate background at the character selection screen, another battle with Aeon, and a final Boss battle with the Time Reaper.

- Hard mode
Successfully complete Story mode on the Normal difficulty to unlock the Hard difficulty.

- Bonus characters
Successfully complete the indicated task to unlock the corresponding character in Story mode:

Carmilla	Successfully complete Story mode with Eric.
Golem	Successfully complete Story mode with Grant.
Aeon	Connect to Castlevania
Shanoa	Connect to Castlevania
Eric	Successfully complete Story mode with Maria.
Maria	Successfully complete Story mode with Alucard.
Death	Successfully complete Story mode with Trevor. Alternately, successfully complete True Story mode with Death.
Grant	Successfully complete Story mode with Sypha.
Dracula	Successfully complete Story mode with Simon, Alucard, Maria, Eric, Carmilla, Grant, Golem, Sypha, Cornell, Trevor, Death, and Shanoa. Alternately, complete True Story mode with Dracula.
Cornell	Successfully complete Story mode with Carmilla.
Sypha	Successfully complete Story mode with Simon.
Trevor	Successfully complete Story mode with Simon, Alucard, Maria, Eric, Carmilla, Grant, Golem, Sypha, and Cornell.

- Brutal mode
Successfully complete Story mode on the Hard difficulty to unlock the Brutal difficulty.