| Sakura (Street Fighter 2) costume for Meg | Successfully complete all the Wii-mote training missions. |
| Chun-Li (Street Fighter) costume for Lisa | Successfully complete Second Shot on all three difficulties. |
| Arthur (Ghosts N' Goblins) costume for Carlos | Successfully complete all Ring Challenges. |
| Pearl Fey (Phoenix Wright) costume for Annie | Successfully complete Tee Shot on all three difficulties. |
| Ryu (Street Fighter) costume for Tony | Successfully complete Approach Shots on all three difficulties. |
| Ken (Street Fighter) costume for Mark | Be victorious in a Friend match. |
| Apollo Justice (Apollo Justice | Ace Attorney) costume for Jack |
| Morrigan (Darkstalkers) costume for Stephanie | Be victorious in a World Play match in Wi-Fi mode. |
| Captain Rose (Zack And Wiki | Quest For Barbaros' Treasure) costume for Marissa |
| Jill Valentine (Resident Evil) costume for Yuki | Successfully complete Putting on all three difficulties. |
| Guile (Street Fighter) costume for Brian | Get a 'Gold' rank on all Mirror Tournament courses. |
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| Zack (Zack And Wiki | Quest For Barbaros' Treasure) costume for Leo |
Be victorious in on a course in Single Player Tournament mode to unlock that course.
Get a 'Gold' rank on all regular Tournament courses to unlock the Pro Tournament.
Overcome a CPU character in Single Player Vs. Match mode to unlock that person as a playable character. Unlock all CPU characters to also unlock your Mii as a playable character in all Single Player modes.
Be victorious in all the Pro Tournament courses to unlock the Mirror Tournament.
